The Antibacterial Activity Mode of Action of Plantaricin YKX against <i>Staphylococcus aureus</i>
Jinjin Pei,
Yigang Huang,
Ting Ren,
Yaodong Guo,
Jun Dang,
Yanduo Tao,
Yonggui Zhang,
A. M. Abd El-Aty
Affiliations
Jinjin Pei
Northwest Institute of Plateau Biology, Chinese Acadamy of Science, Xining 530001, China
Yigang Huang
Shaanxi Province Key Laboratory of Bioresources, 2011 QinLing-Bashan Mountains Bioresources Comprehensive Development C. I. C., Qinba State Key Laboratory of Biological Resources and Ecological Environment, College of Bioscience and Bioengineering, Shaanxi University of Technology, Hanzhong 723001, China
Ting Ren
Shaanxi Province Key Laboratory of Bioresources, 2011 QinLing-Bashan Mountains Bioresources Comprehensive Development C. I. C., Qinba State Key Laboratory of Biological Resources and Ecological Environment, College of Bioscience and Bioengineering, Shaanxi University of Technology, Hanzhong 723001, China
Yaodong Guo
College of Health and Management, Shangluo University, Shangluo 724900, China
Jun Dang
Northwest Institute of Plateau Biology, Chinese Acadamy of Science, Xining 530001, China
Yanduo Tao
Northwest Institute of Plateau Biology, Chinese Acadamy of Science, Xining 530001, China
Yonggui Zhang
Shaanxi Province Key Laboratory of Bioresources, 2011 QinLing-Bashan Mountains Bioresources Comprehensive Development C. I. C., Qinba State Key Laboratory of Biological Resources and Ecological Environment, College of Bioscience and Bioengineering, Shaanxi University of Technology, Hanzhong 723001, China
A. M. Abd El-Aty
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt
We aimed to evaluate the inhibitory effect and mechanism of plantaricin YKX on S. aureus. The mode of action of plantaricin YKX against the cells of S. aureus indicated that plantaricin YKX was able to cause the leakage of cellular content and damage the structure of the cell membranes. Additionally, plantaricin YKX was also able to inhibit the formation of S. aureus biofilms. As the concentration of plantaricin YKX reached 3/4 MIC, the percentage of biofilm formation inhibition was over 50%. Fluorescent dye labeling combined with fluorescence microscopy confirmed the results. Finally, the effect of plantaricin YKX on the AI-2/LuxS QS system was investigated. Molecular docking predicted that the binding energy of AI-2 and plantaricin YKX was −4.7 kcal/mol and the binding energy of bacteriocin and luxS protein was −183.701 kcal/mol. The expression of the luxS gene increased significantly after being cocultured with plantaricin YKX, suggesting that plantaricin YKX can affect the QS system of S. aureus.
