Scientific Reports (Oct 2023)

A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme

  • Lopamudra Roy,
  • Susmita Mondal,
  • Neha Bhattacharyya,
  • Ria Ghosh,
  • Amrita Banerjee,
  • Soumendra Singh,
  • Arpita Chattopadhyay,
  • Saleh A. Ahmed,
  • Rabab S. Jassas,
  • Munirah M. Al-Rooqi,
  • Ziad Moussa,
  • Ismail I. Althagafi,
  • Debasish Bhattacharya,
  • Kallol Bhattacharya,
  • Asim Kumar Mallick,
  • Samir Kumar Pal

DOI
https://doi.org/10.1038/s41598-023-44011-y
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 15

Abstract

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Abstract The recent prediction of diabetes to be a global pandemic invites a detection strategy preferably non-invasive, and bloodless to manage the disease and the associated complications. Here, we have synthesized chitosan polymer functionalized, organic–inorganic bio-compatible nano-hybrids of Mn3O4 nanoparticles, and characterized it by utilizing several optical methodologies for the structural characterization which shows the Michaelis Menten (MM) kinetics for glucose and alpha-amylase protein (well-known diabetes biomarkers). We have also studied the potentiality for the detection of alpha-amylase in human salivary secretion which is reported to be strongly correlated with uncontrolled hyperglycemia. Finally, we have developed a prototype for the measurement of glucose (LOD of 0.38 mg/dL, LOQ of 1.15 mg/dL) and HbA1c (LOD of 0.15% and LOQ of 0.45%) utilizing the basic knowledge in the study for the detection of uncontrolled hyperglycemia at the point-of-care. With the limited number of clinical trials, we have explored the potential of our work in combating the diabetic pandemic across the globe in near future.