Diagnostic sérologique des isolats soudano-sahéliens du virus de la panachure jaune du riz (Rice Yellow Mottle Virus, RYMV)

Abstract

Serological Diagnosis of Sudano-Sahelian Isolates of Rice Yellow Mottle Virus (RYMV). Rice Yellow Mottle Virus (RYMV) is the most damaging virus infecting rice in Africa. Serology is a suitable detection method for RYMV but most available anti-RYMV polyclonal antibodies react poorly with some isolates of the virus, which undermine the reliability of the method. A broad-spectrum polyclonal antibody was raised against Rice Yellow Mottle Virus (RYMV) in order to improve the serological detection of the virus. This antibody was used to diagnose the virus in field samples collected from both irrigated and upland rice and wild host plants in five West and Central African countries (Burkina Faso, Cameroon, Chad, Mali and Togo). RYMV was readily detected in about 500 samples whereas a new natural host species (Eragrostis atrovirens) collected from Mali was identified. The viral isolates gathered in this study constitute the starting point for a reference collection of plant viruses in the Sudano-Sahelian region. This collection will be particularly useful for the assessment of RYMV variability which is a key factor in developing rice varieties with stable resistance to the virus.

Keywords

• Phytovirus
• Broad
• spectrum Polyclonal Antibody
• Sudano
• Sahelian zone
• Burkina Faso