CELL TECHNOLOGIES IN THE CORRECTION OF METABOLIC DISORDERS OF ERYTHROCYTES IN ACUTE TOXIC LIVER INJURY

Abstract

Background: Establishing effective use of allogeneic hepatocytes and Mexidol in the correction of the content of protein and erythrocyte membrane lipids and metabolic indicators in acute liver toxicity. Materials and methods: Studies conducted on 65 male rats Wistar. Acute liver toxicity was modeled by intramuscular injection of carbon tetrachloride. Isolation of allogeneic hepatocytes from animals 5–6 days after birth was made by the method M.N. Berry, D.S. Friend. Meksidol administered simultaneously with the toxicant, five times, after 24 hours, intraperitoneally at 50 mg/kg, alone or with allogeneic hepatocytes. Results: The article presents the data on violations of proteins and lipids contents of erythrocytes membranes and erythrocytes metabolism values in experimental animals under the condition of acute toxic liver injury (ATLI), caused by the introduction of carbon tetrachloride. This scientific work reveals the effectiveness of allogenic hepatocytes and Mexidol using for the correction of abnormal values of lipid spectrum of erythrocytes membranes and erythrocytes metabolism indexes in animals with acute toxic liver injury. Conclusion: Effect of Mexidol in acute liver injury caused by toxic normalization of lipid metabolism in the cell membrane and thus membrane protein spectrum. Scope of the results. The results obtained can be used to develop effective ways of correction of metabolic abnormalities in different types of liver disease.

Keywords

• острая токсическая гепатопатия
• эритроциты
• аллогенные гепатоциты
• мексидол