Metabolism of proinflammatory cytokine interleukine-ip and oxidant activity of neutrophils in various forms of chronic bronchitis

Abstract

Aim. To study content of interleukin-ip (IL-ip) and free radical oxidation in neutrophils of blood, saliva and bronchoalveolar lavage (BAL) in chronic bronchitis for prediction of obstructive defects. Material and methods. Enzyme immunoassay was used to measure IL-ip in the blood, saliva, BAL of 49 males with chronic bronchitis aged 39- 72 years. Functional ability of the neutrophils to generate free oxygen radicals was assessed by luminol-dependent chemiluminescence. Results. Patients with chronic obstructive bronchitis exhibited excessive accumulation of IL-ip in the blood and BAL, enhanced activity of hematosalivary barrier for IL-lp and production of active oxygen radicals by systemic and tissue neutrophils. Conclusion. Elevated concentration of systemic and tissue IL-lp, activation of free radical oxidation by blood neutrophils and BAL, enhancement of hematosalivary barrier for IL-ip can be considered as prognosticate unfavourable factors of bronchial obstruction in patients with chronic bronchitis.

Keywords

• chronic bronchitis
• bronchial obstruction
• interleukin-lp
• neutrophils
• free radical oxidation