Human breast milk concentration of neopterin at various stages of lactation and during a single feeding
Plata-Nazar Katarzyna,
Woś-Wasilewska Ewa,
Szlagatys-Sidorkiewcz Agnieszka,
Łuczak Grażyna,
Zagierski Maciej,
Martysiak-Żurowska Dorota,
Kamińska Barbara
Affiliations
Plata-Nazar Katarzyna
Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, 1-6 Nowe Ogrody St., 80-803 Gdansk, Poland
Woś-Wasilewska Ewa
Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, 1-6 Nowe Ogrody St., 80-803 Gdansk, Poland
Szlagatys-Sidorkiewcz Agnieszka
Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, 1-6 Nowe Ogrody St., 80-803 Gdansk, Poland
Łuczak Grażyna
Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, 1-6 Nowe Ogrody St., 80-803 Gdansk, Poland
Zagierski Maciej
Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, 1-6 Nowe Ogrody St., 80-803 Gdansk, Poland
Martysiak-Żurowska Dorota
Chemical Faculty, Department of Food Chemistry, Technology and Biotechnology, Gdansk University of Technology, 11/12 G. Narutowicza St., 80-233 Gdansk, Poland
Kamińska Barbara
Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, 1-6 Nowe Ogrody St., 80-803 Gdansk, Poland
The aim of this work was to determine the physiological level of neopterin in human breast milk, and to study its variability depending on the duration of a single feeding and the lactation stage. Breast milk samples from 74 women were collected between 2 and 4 days after delivery, and at 15, 30, and 90 days after delivery. Additionally, breast milk samples from eight women were collected before and after 7 and 15 min of breastfeeding. The concentration of neopterin in breast milk was determined by an immunoenzymatic assay. The range of breast milk neopterin concentration at various stages of lactation amounted to 15.4–19.2 nmol/L at 2–4 days after delivery, 20.2–23.0 nmol/L at day 15, 20.8–24.5 nmol/L at day 30, and 16.9–20.4 nmol/L at day 90. The level of neopterin 2–4 days after delivery was significantly lower than that at days 15 and 30; moreover, the concentration of neopterin at day 30 was significantly higher than that at day 90. No significant differences were documented between neopterin concentrations at various phases of a single feeding. While the breast milk concentration of neopterin changes depending on the stage of lactation, it remains stable throughout a single feeding.
