Role of HIKESHI on Hyperthermia for Castration-Resistant Prostate Cancer and Application of a Novel Magnetic Nanoparticle with Carbon Nanohorn for Magnetic Hyperthermia
Takashi Nagai,
Noriyasu Kawai,
Masakazu Gonda,
Keitaro Iida,
Toshiki Etani,
Daichi Kobayashi,
Taku Naiki,
Aya Naiki-Ito,
Ryosuke Ando,
Sataro Yamaguchi,
Yuto Sugahara,
Sakyo Ueno,
Kaname Tsutsumiuchi,
Toyoko Imae,
Takahiro Yasui
Affiliations
Takashi Nagai
Department of Nephron-Urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Aichi, Japan
Noriyasu Kawai
Department of Nephron-Urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Aichi, Japan
Masakazu Gonda
Department of Nephron-Urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Aichi, Japan
Keitaro Iida
Department of Nephron-Urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Aichi, Japan
Toshiki Etani
Department of Nephron-Urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Aichi, Japan
Daichi Kobayashi
Department of Nephron-Urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Aichi, Japan
Taku Naiki
Department of Nephron-Urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Aichi, Japan
Aya Naiki-Ito
Experimental Pathology and Tumor Biology, Nagoya City University, Nagoya 467-8601, Aichi, Japan
Ryosuke Ando
Department of Nephron-Urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Aichi, Japan
Sataro Yamaguchi
Center of Applied Superconductivity and Energy Research (CASER), Chubu University, 1200 Matsumoto, Kasugai 487-8501, Aichi, Japan
Yuto Sugahara
College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai 487-8501, Aichi, Japan
Sakyo Ueno
College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai 487-8501, Aichi, Japan
Kaname Tsutsumiuchi
College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai 487-8501, Aichi, Japan
Toyoko Imae
Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan
Takahiro Yasui
Department of Nephron-Urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Aichi, Japan
The prognosis of castration-resistant prostate cancer (CRPC) is technically scarce; therefore, a novel treatment for CRPC remains warranted. To this end, hyperthermia (HT) was investigated as an alternative therapy. In this study, the analysis focused on the association between CRPC and heat shock protein nuclear import factor “hikeshi (HIKESHI)”, a factor of heat tolerance. Silencing the HIKESHI expression of 22Rv1 cells (human CRPC cell line) treated with siRNAs inhibited the translocation of heat shock protein 70 from the cytoplasm to the nucleus under heat shock and enhanced the effect of hyperthermia. Moreover, a novel magnetic nanoparticle was developed via binding carbon nanohorn (CNH) and iron oxide nanoparticle (IONP) with 3-aminopropylsilyl (APS). Tumor-bearing model mice implanted with 22 Rv1 cells were examined to determine the effect of magnetic HT (mHT). We locally injected CNH-APS-IONP into the tumor, which was set under an alternative magnetic field and showed that tumor growth in the treatment group was significantly suppressed compared with other groups. This study suggests that HIKESHI silencing enhances the sensitivity of 22Rv1 cells to HT, and CNH-APTES-IONP deserves consideration for mHT.
