mSphere (Apr 2021)

Genome-Based Targeted Sequencing as a Reproducible Microbial Community Profiling Assay

  • Jacquelynn Benjamino,
  • Benjamin Leopold,
  • Daniel Phillips,
  • Mark D. Adams

DOI
https://doi.org/10.1128/mSphere.01325-20
Journal volume & issue
Vol. 6, no. 2

Abstract

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New methods for profiling the microbial communities can create new approaches to understanding the composition and function of those communities. In this study, we combined bacterial genome-specific probe design with a highly multiplexed single primer extension reaction as a new method to profile microbial communities, using stool from various mouse strains as a test case.