Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry
Eugene B. Postnikov,
Michał Wasiak,
Mariola Bartoszek,
Justyna Polak,
Andrey Zyubin,
Anastasia I. Lavrova,
Mirosław Chora̧żewski
Affiliations
Eugene B. Postnikov
Theoretical Physics Department, Kursk State University, Radishcheva St. 33, 305000 Kursk, Russia
Michał Wasiak
Department of Physical Chemistry, University of Lódź, ul. Pomorska 165, 90-236 Lódź, Poland
Mariola Bartoszek
Institute of Chemistry, University of Silesia in Katowice, ul. Szkolna 9, 40-006 Katowice, Poland
Justyna Polak
Institute of Chemistry, University of Silesia in Katowice, ul. Szkolna 9, 40-006 Katowice, Poland
Andrey Zyubin
Sophya Kovalevskaya North-West Mathematical Research Center, Immanuel Kant Baltic Federal University, Nevskogo St. 14, 236041 Kaliningrad, Russia
Anastasia I. Lavrova
Sophya Kovalevskaya North-West Mathematical Research Center, Immanuel Kant Baltic Federal University, Nevskogo St. 14, 236041 Kaliningrad, Russia
Mirosław Chora̧żewski
Institute of Chemistry, University of Silesia in Katowice, ul. Szkolna 9, 40-006 Katowice, Poland
In this work, we review some physical methods of macroscopic experiments, which have been recently argued to be promising for the acquisition of valuable characteristics of biomolecular structures and interactions. The methods we focused on are electron paramagnetic resonance spectroscopy, Raman spectroscopy, and differential scanning calorimetry. They were chosen since it can be shown that they are able to provide a mutually complementary picture of the composition of cellular envelopes (with special attention paid to mycobacteria), transitions between their molecular patterning, and the response to biologically active substances (reactive oxygen species and their antagonists—antioxidants—as considered in our case study).
