Comparative fitness analysis of D-cycloserine resistant mutants reveals both fitness-neutral and high-fitness cost genotypes

Dimitrios Evangelopoulos; Gareth A. Prosser; Angela Rodgers; Belinda M. Dagg; Bhagwati Khatri; Mei Mei Ho; Maximiliano G. Gutierrez; Teresa Cortes; Luiz Pedro S. de Carvalho

doi:10.1038/s41467-019-12074-z

Nature Communications (Sep 2019)

Comparative fitness analysis of D-cycloserine resistant mutants reveals both fitness-neutral and high-fitness cost genotypes

Dimitrios Evangelopoulos,
Gareth A. Prosser,
Angela Rodgers,
Belinda M. Dagg,
Bhagwati Khatri,
Mei Mei Ho,
Maximiliano G. Gutierrez,
Teresa Cortes,
Luiz Pedro S. de Carvalho

Affiliations

Dimitrios Evangelopoulos: Mycobacterial Metabolism and Antibiotic Research Laboratory, The Francis Crick Institute
Gareth A. Prosser: Mycobacterial Metabolism and Antibiotic Research Laboratory, The Francis Crick Institute
Angela Rodgers: Mycobacterial Metabolism and Antibiotic Research Laboratory, The Francis Crick Institute
Belinda M. Dagg: Bacteriology Division, National Institute for Biological Standards and Control (MHRA-NIBSC), Blanche Lane, South Mimms
Bhagwati Khatri: Bacteriology Division, National Institute for Biological Standards and Control (MHRA-NIBSC), Blanche Lane, South Mimms
Mei Mei Ho: Bacteriology Division, National Institute for Biological Standards and Control (MHRA-NIBSC), Blanche Lane, South Mimms
Maximiliano G. Gutierrez: Host-Pathogen Interactions in Tuberculosis Laboratory, The Francis Crick Institute
Teresa Cortes: Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine
Luiz Pedro S. de Carvalho: Mycobacterial Metabolism and Antibiotic Research Laboratory, The Francis Crick Institute

DOI: https://doi.org/10.1038/s41467-019-12074-z
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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D-cycloserine (DCS) has been used for decades to treat Mycobacterium tuberculosis (Mtb) but resistance is rarely observed in clinical isolates. Here, the authors report ultra-low rate of emergence of resistance mutations as the underlying mechanism of DCS resistance evasion in Mtb.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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