Lipid droplets imaging with three-photon microscopy

Mubin He; Hojeong Park; Guangle Niu; Qiming Xia; Hequn Zhang; Ben Zhong Tang; Jun Qian

doi:10.1142/S179354582250033X

Journal of Innovative Optical Health Sciences (Jul 2023)

Lipid droplets imaging with three-photon microscopy

Mubin He,
Hojeong Park,
Guangle Niu,
Qiming Xia,
Hequn Zhang,
Ben Zhong Tang,
Jun Qian

Affiliations

Mubin He: State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou 310058, P. R. China
Hojeong Park: Department of Chemistry, The Hong Kong Branch of Chinese, National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, P. R. China
Guangle Niu: Shenzhen Research Institute of Shandong University, Shenzhen 518057, P. R. China
Qiming Xia: Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, P. R. China
Hequn Zhang: State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou 310058, P. R. China
Ben Zhong Tang: School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P. R. China
Jun Qian: State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou 310058, P. R. China

DOI: https://doi.org/10.1142/S179354582250033X
Journal volume & issue: Vol. 16, no. 04

Abstract

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Lipid droplets (LDs) participate in many physiological processes, the abnormality of which will cause chronic diseases and pathologies such as diabetes and obesity. It is crucial to monitor the distribution of LDs at high spatial resolution and large depth. Herein, we carried three-photon imaging of LDs in fat liver. Owing to the large three-photon absorption cross-section of the luminogen named NAP-CF3 ([Formula: see text][Formula: see text]cm6 s2), three-photon fluorescence fat liver imaging reached the largest depth of 80[Formula: see text][Formula: see text]m. Fat liver diagnosis was successfully carried out with excellent performance, providing great potential for LDs-associated pathologies research.

Published in Journal of Innovative Optical Health Sciences

ISSN: 1793-5458 (Print); 1793-7205 (Online)
Publisher: World Scientific Publishing
Country of publisher: Singapore
LCC subjects: Technology; Science: Physics: Optics. Light
Website: http://www.worldscientific.com/worldscinet/jiohs

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