Nature Communications (Mar 2018)

An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations

  • Xiaonan Liu,
  • Kari Salokas,
  • Fitsum Tamene,
  • Yaming Jiu,
  • Rigbe G. Weldatsadik,
  • Tiina Öhman,
  • Markku Varjosalo

DOI
https://doi.org/10.1038/s41467-018-03523-2
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 16

Abstract

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AP-MS and BioID provide complementary insights into cellular protein interaction networks. To facilitate their combined use, the authors here present an AP-MS- and BioID-compatible affinity tag, enabling efficient determination of cellular protein locations and interaction distances.