Microorganisms (Feb 2023)

Detection of Oxacillin/Cefoxitin Resistance in <i>Staphylococcus aureus</i> Present in Recurrent Tonsillitis

  • Aline Cristine Magalhães Costa Messias,
  • Aline Rodrigues Gama,
  • Letícia Suriano de Almeida Prado,
  • Paulo Alex Neves da Silva,
  • Arlindo Rodrigues Galvão Filho,
  • Clarimar José Coelho,
  • Melissa Ameloti Gomes Avelino,
  • José Daniel Gonçalves Vieira,
  • Lilian Carla Carneiro

DOI
https://doi.org/10.3390/microorganisms11030615
Journal volume & issue
Vol. 11, no. 3
p. 615

Abstract

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Background: Recurrent tonsillitis is one of the most common diseases in childhood, caused many times by ß-lactam-resistant S. aureus. The objective of this study was to investigate an alternative method to identify resistance to oxacillin/cefoxitin in S. aureus from hospitalized children with recurrent tonsillitis. Methods: The samples of S. aureus came from patients with recurrent tonsillitis and were used in 16S rRNA sequencing and an antibiogram test for identification and verifying resistance, after which HSI methodology were applied for separation of S. aureus resistances. Results: The S. aureus isolated showed sensitivity to oxacillin/cefoxitin and the diagnostic images show a visual description of the resistance different groups formed, that may be related to sensitivity and resistance to oxacillin/cefoxitin, characterizing the MRSA S. aureus. Conclusions: Samples that showed phenotypic resistance to oxacillin/cefoxitin were clearly separated from samples that did not show this resistance. A PLS-DA model predicted the presence of resistance to oxacillin/cefoxitin in S. aureus samples and it was possible to observe the pixels classified as MRSA. The HSI was able to successfully discriminate samples in replicas that were sensitive and resistant, based on the calibration model it received.

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