Tolerance to Imazamox Herbicide Found after Screening of Advanced Generation Lentil Mutant Genotypes

Abstract

In Turkey, one of the essential grain legumes is lentil. It is usually perceived as a weak competitor with weeds. The research objective was to determine the tolerance of selected 145 mutagenized lentil genotypes at M5 generation to imazamox herbicide including 139 M5 lentil genotypes derived from Ethyl Methane Sulfonate (EMS) mutagenized seeds of cultivar Firat-87 and 6 control lentil cultivars were screened for imazamox herbicide tolerance. Experiments were carried out in the greenhouse and field. Herbicide was applied at 150% of the recommended dose of (100 ml/ha, or 40 g a.i/ha) imazamox when the plants were between 5 - 6 node stage. The response of the genotypes to the herbicide was evaluated by measuring the plant height as a sign of the growth and also by visual scoring of foliar damage with a 1 to 5 scale at 45 and 60 days after a spraying in the field experiment and at 30 and 60 days after a spraying in the greenhouse experiment. The genotypes were categorized based on their reactions to herbicides as highly tolerant, tolerant, moderately tolerant, sensitive, and highly sensitive. The results showed significant differences among the genotypes for tolerance to the herbicide. At 60 days after spray, most of the genotypes showed some of the recoveries in both experiments. Five genotypes (IMI-124, IMI-128, IMI-130, IMI-138, and IMI-139), displayed high herbicide tolerance in both experiments. The tolerant genotypes can be exploited in future breeding programs for improving herbicide tolerant lentil varieties.

Keywords

• lentil
• ethyl methane sulfonate (ems)
• imazamox tolerance