Alternative Rapid Methods for Coverslip Removal: A Comparative Study

Abstract

Introduction: In 21st century, the focus is to develop method which yields outcome in shorter tenure, and are rapid, safe and obtain quicker results. In this study, non-traditional methods were used for removal of coverslip and was compared with traditional methods. Aim: To compare the time taken for coverslip removal of old faded slides with five different methods. Materials and Methods: Faded slides of one year to more than 10-year-old were included in the study. Total of 90 slides were subjected to five different methods of coverslip removal like xylene at room temperature, xylene at 56° Celsius (C), Petrol, Diesel and Freezing method. Time taken for each method was noted. Results: The mean time taken for removal of coverslip was least with freezing method followed by xylene at 56°C, Petrol, xylene at room temperature and diesel. Conclusion: Alternative methods are safe, rapid and has less turn around time compared to routine use of xylene. Hence, can be successfully used for removal of coverslip.

Keywords

• freezing
• petrol
• restaining
• xylene