Frontiers in Physiology (Sep 2020)
Identifying the Related Genes of Muscle Growth and Exploring the Functions by Compensatory Growth in Mandarin Fish (Siniperca chuatsi)
- Xuange Liu,
- Xuange Liu,
- Xuange Liu,
- Shuang Zeng,
- Shuang Zeng,
- Shuang Zeng,
- Shuang Liu,
- Shuang Liu,
- Shuang Liu,
- Gongpei Wang,
- Gongpei Wang,
- Gongpei Wang,
- Han Lai,
- Han Lai,
- Han Lai,
- Xiaopin Zhao,
- Xiaopin Zhao,
- Xiaopin Zhao,
- Sheng Bi,
- Sheng Bi,
- Sheng Bi,
- Dingli Guo,
- Dingli Guo,
- Dingli Guo,
- Xiaoli Chen,
- Xiaoli Chen,
- Xiaoli Chen,
- Huadong Yi,
- Huadong Yi,
- Huadong Yi,
- Yuqin Su,
- Yuqin Su,
- Yuqin Su,
- Yong Zhang,
- Yong Zhang,
- Yong Zhang,
- Guifeng Li,
- Guifeng Li,
- Guifeng Li
Affiliations
- Xuange Liu
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Xuange Liu
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Xuange Liu
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Shuang Zeng
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Shuang Zeng
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Shuang Zeng
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Shuang Liu
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Shuang Liu
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Shuang Liu
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Gongpei Wang
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Gongpei Wang
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Gongpei Wang
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Han Lai
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Han Lai
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Han Lai
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Xiaopin Zhao
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Xiaopin Zhao
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Xiaopin Zhao
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Sheng Bi
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Sheng Bi
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Sheng Bi
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Dingli Guo
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Dingli Guo
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Dingli Guo
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Xiaoli Chen
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Xiaoli Chen
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Xiaoli Chen
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Huadong Yi
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Huadong Yi
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Huadong Yi
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Yuqin Su
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Yuqin Su
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Yuqin Su
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Yong Zhang
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Yong Zhang
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Yong Zhang
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- Guifeng Li
- Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Guifeng Li
- Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, China
- Guifeng Li
- Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China
- DOI
- https://doi.org/10.3389/fphys.2020.553563
- Journal volume & issue
-
Vol. 11
Abstract
How organisms display many different biochemical, physiological processes through genes expression and regulatory mechanisms affecting muscle growth is a central issue in growth and development. In Siniperca chuatsi, the growth-related genes and underlying relevant mechanisms are poorly understood, especially for difference of body sizes and compensatory growth performance. Muscle from 3-month old individuals of different sizes was used for transcriptome analysis. Results showed that 8,942 different expression genes (DEGs) were identified after calculating the RPKM. The DEGs involved in GH-IGF pathways, protein synthesis, ribosome synthesis and energy metabolisms, which were expressed significantly higher in small individuals (S) than large fish (L). In repletion feeding and compensatory growth experiments, eight more significant DEGs were used for further research (GHR2, IGFR1, 4ebp, Mhc, Mlc, Myf6, MyoD, troponin). When food was plentiful, eight genes participated in and promoted growth and muscle synthesis, respectively. Starvation can be shown to inhibit the expression of Mhc, Mlc and troponin, and high expression of GHR2, IGFR1, and 4ebp inhibited growth. Fasting promoted the metabolic actions of GHR2, IGFR1, and 4ebp rather than the growth-promoting actions. MyoD can sense and regulate the hunger, which also worked with Mhc and Mlc to accelerate the compensatory growth of S. chuatsi. This study is helpful to understand the regulation mechanisms of muscle growth-related genes. The elected genes will contribute to the selective breeding in future as candidate genes.
Keywords
