Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo

Chuan Xu; Hong Wu; Yiqiang Liu; Feifei Li; Rajesh Kumar Manne; Hui-Kuan Lin

STAR Protocols (Mar 2023)

Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo

Chuan Xu,
Hong Wu,
Yiqiang Liu,
Feifei Li,
Rajesh Kumar Manne,
Hui-Kuan Lin

Affiliations

Chuan Xu: Department of Oncology, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China; Integrative Cancer Center & Cancer Clinical Research Center, Sichuan Cancer Hospital & Institute Sichuan Cancer Center, School of Medicine University of Electronic Science and Technology of China, Chengdu, Sichuan 610047, China; Department of Cancer Biology, Department of Cancer Biology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA; Corresponding author
Hong Wu: Department of Oncology, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China; Integrative Cancer Center & Cancer Clinical Research Center, Sichuan Cancer Hospital & Institute Sichuan Cancer Center, School of Medicine University of Electronic Science and Technology of China, Chengdu, Sichuan 610047, China
Yiqiang Liu: Department of Oncology, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China; Integrative Cancer Center & Cancer Clinical Research Center, Sichuan Cancer Hospital & Institute Sichuan Cancer Center, School of Medicine University of Electronic Science and Technology of China, Chengdu, Sichuan 610047, China
Feifei Li: Department of Oncology, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China; Integrative Cancer Center & Cancer Clinical Research Center, Sichuan Cancer Hospital & Institute Sichuan Cancer Center, School of Medicine University of Electronic Science and Technology of China, Chengdu, Sichuan 610047, China
Rajesh Kumar Manne: Department of Cancer Biology, Department of Cancer Biology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
Hui-Kuan Lin: Department of Cancer Biology, Department of Cancer Biology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA; Corresponding author

Journal volume & issue: Vol. 4, no. 1
p. 101940

Abstract

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Summary: Here, we present optimized approaches to identify the efficiency of cancer cell phagocytosis by macrophages in vitro and in vivo. We describe the preparation and co-culture of macrophages and cancer cells, followed by in vitro phagocytosis assay using flow cytometry and confocal microscopy, respectively. We then detail the establishment of xenograft tumor mouse model and the in vivo detecting of phagocytosis efficiency by flow cytometry and qRT-PCR. This protocol provides a convenient way to assess macrophage-mediated phagocytosis of cancer cells.For complete details on the use and execution of this protocol, please refer to Xu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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