Bakteri dalam Plak Gigi dan Saliva Penderita Diabetes Tipe 2, dan Hubungannya dengan Status Agregasi Trombosit

Abstract

The occurrence of coronary atherosclerosis and acute myocardianl ischemia due to the hyperaggregation of platelets, caused the death of 75-80% of type 2 diabetics. In an invitro study, the aggregation of platelets was influenced by the "Platelet Aggregation Associated Protein" on the surface of dental plaque bacteria, S. sanguis. The aim of the study was to identify bacteria in the supragingival plaque and saliva of type 2 diabetics, and their relationship to the aggregation of platelets. Dental plaque and salivary gland samples from 5 type 2 diabetics were diluted serially, and inoculated in Mitis Salivarius medium. The morphologie of the Colony Forming Units (CFU) Growth were analysed, identified physiologically and biochemically with Api 20 Strep subtrates. Aggregation of platelets of the blood were analysed with Adenosin Di Phosphate. The result of the study showed 22 colonies (CFU), in 6 morphology types. Three morphologytypes were identified as A viridans, the other 3 as Str. acidominus, Lc. lactis lactis, and E. avium. One morphology type of A. viridans, Str .acidominus, Lc. lactis lactis and E. avium were found in subjects with hypoaggregation; 2 morphology types of A. viridans were found in subjects with normal aggregation. It is presumed that type 2 diabetics with Str. acidominus, Lc. lactis actis, E. avium, and certain morphology type of A. viridans in their dental plaque and saliva, hve hyperaggregation of platelet diabetics with two morphology types of A. viridans have normal aggregation of platelets.