Green Synthesis of Silver Nitrate Nanoparticles from E. coli and Evaluation of Their Inhibitory Effectiveness against E. coli, S. aureus, and B. subtilis

Abstract

this study investigated the environmentally friendly production of silver nitrate nanoparticles (Agno3 NPs) utilizing E.coli and assesses their ability to kill harmful bacteria such as E.coli , Staphylococcus aureus , and bacillus subtilis employing biological processes for nanoparticles synthesis offers both ecological advantages and improved compatibility with biological system the biogenic synthesis entailed cultivating E.coli in tryptic soya broth, and subsequently treating it with silver nitrate solution to generate AgNPs the produced nanoparticles underwent characterization using scanning electrone microscope (SEM), X-ray diffraction (XRD) and energy dispersive X ray (EDX) examination the antibacterial efficacy was evaluated by quantifying the zone of inhibition diameter against the microorganisms being tested, indicating that higher quantities of AgNPS where associated with greater antibacterial potency . this study emphasizes the possibility of utilizing biologically produced nanoparticles in medical applications, namely as a substitute for conventional antibacterial treatment which frequently encounter challenges related to resistance

Keywords

• Green synthesis, Silver nanoparticles, X-ray diffraction, scanning electron microscopy