Nature Communications (Nov 2022)
PCGF1-PRC1 links chromatin repression with DNA replication during hematopoietic cell lineage commitment
- Junichiro Takano,
- Shinsuke Ito,
- Yixing Dong,
- Jafar Sharif,
- Yaeko Nakajima-Takagi,
- Taichi Umeyama,
- Yong-Woon Han,
- Kyoichi Isono,
- Takashi Kondo,
- Yusuke Iizuka,
- Tomohiro Miyai,
- Yoko Koseki,
- Mika Ikegaya,
- Mizuki Sakihara,
- Manabu Nakayama,
- Osamu Ohara,
- Yoshinori Hasegawa,
- Kosuke Hashimoto,
- Erik Arner,
- Robert J. Klose,
- Atsushi Iwama,
- Haruhiko Koseki,
- Tomokatsu Ikawa
Affiliations
- Junichiro Takano
- Laboratory for Immune Regeneration, RIKEN Center for Integrative Medical Sciences (RIKEN-IMS)
- Shinsuke Ito
- Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences
- Yixing Dong
- Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences
- Jafar Sharif
- Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences
- Yaeko Nakajima-Takagi
- Division of Stem Cell and Molecular Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, University of Tokyo
- Taichi Umeyama
- Laboratory for Microbiome Sciences, RIKEN-IMS
- Yong-Woon Han
- Laboratory for Integrative Genomics, RIKEN-IMS
- Kyoichi Isono
- Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences
- Takashi Kondo
- Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences
- Yusuke Iizuka
- Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences
- Tomohiro Miyai
- Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences
- Yoko Koseki
- Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences
- Mika Ikegaya
- Laboratory for Immune Regeneration, RIKEN Center for Integrative Medical Sciences (RIKEN-IMS)
- Mizuki Sakihara
- Division of Immunology and Allergy, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda
- Manabu Nakayama
- Chromosome Engineering Team, Department of Technology Development, Kazusa DNA Research Institute
- Osamu Ohara
- Chromosome Engineering Team, Department of Technology Development, Kazusa DNA Research Institute
- Yoshinori Hasegawa
- Chromosome Engineering Team, Department of Technology Development, Kazusa DNA Research Institute
- Kosuke Hashimoto
- Laboratory of Computational Biology, Institute for Protein Research
- Erik Arner
- Laboratory for Applied Regulatory Genomics Network Analysis, RIKEN-IMS
- Robert J. Klose
- Department of Biochemistry, University of Oxford
- Atsushi Iwama
- Division of Stem Cell and Molecular Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, University of Tokyo
- Haruhiko Koseki
- Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences
- Tomokatsu Ikawa
- Laboratory for Immune Regeneration, RIKEN Center for Integrative Medical Sciences (RIKEN-IMS)
- DOI
- https://doi.org/10.1038/s41467-022-34856-8
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 19
Abstract
Here the authors show that a Polycomb Repressive Complex 1 (PRC1) containing PCGF1 prevents excessive loading of transcriptional activators and chromatin remodelers on nascent DNA, allowing proper deposition of nucleosomes immediately after the passage of the DNA replication fork to optimize downstream chromatin configurations in hematopoietic stem and progenitor cells (HSPCs).
