Association Mapping for Quantitative Trait Loci Controlling Superoxide Dismutase, Flavonoids, Anthocyanins, Carotenoids, γ-Oryzanol and Antioxidant Activity in Rice
Ramakrushna Bastia,
Elssa Pandit,
Priyadarsini Sanghamitra,
Saumya Ranjan Barik,
Deepak Kumar Nayak,
Auromira Sahoo,
Arpita Moharana,
Jitendriya Meher,
Prasanta K. Dash,
Reshmi Raj,
Binod Kumar Jena,
Kartik Chandra Pradhan,
Devraj Lenka,
Nabaneeta Basak,
Srikanta Lenka,
Sharat Kumar Pradhan
Affiliations
Ramakrushna Bastia
ICAR-National Rice Research Institute, Cuttack 753006, India
Elssa Pandit
ICAR-National Rice Research Institute, Cuttack 753006, India
Priyadarsini Sanghamitra
ICAR-National Rice Research Institute, Cuttack 753006, India
Saumya Ranjan Barik
ICAR-National Rice Research Institute, Cuttack 753006, India
Deepak Kumar Nayak
ICAR-National Rice Research Institute, Cuttack 753006, India
Auromira Sahoo
ICAR-National Rice Research Institute, Cuttack 753006, India
Arpita Moharana
ICAR-National Rice Research Institute, Cuttack 753006, India
Jitendriya Meher
ICAR-National Rice Research Institute, Cuttack 753006, India
Prasanta K. Dash
ICAR-National Institute for Plant Biotechnology, New Delhi 110012, India
Reshmi Raj
ICAR-National Rice Research Institute, Cuttack 753006, India
Binod Kumar Jena
KVK, OUAT, Rayagada 765022, India
Kartik Chandra Pradhan
College of Agriculture, OUAT, Bhubaneswar 751003, India
Devraj Lenka
College of Agriculture, OUAT, Bhubaneswar 751003, India
Nabaneeta Basak
ICAR-National Rice Research Institute, Cuttack 753006, India
Srikanta Lenka
ICAR-National Rice Research Institute, Cuttack 753006, India
Sharat Kumar Pradhan
ICAR-National Rice Research Institute, Cuttack 753006, India
Antioxidant-rich rice is a cheaper way to solve stress-related disorders and other health benefits for the global rice-eating population. Five antioxidant traits, namely, superoxide dismutase, flavonoids, anthocyanins, γ-oryzanol and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) activity were mapped using a representative panel population through association mapping. Potential landraces carrying multiple antioxidant compounds were identified from the population. The population represented four genetic groups and correspondence for presence of antioxidants traits in each group was noticed. The population showed linkage disequilibrium for the studied traits based on the Fst values. A total of 14 significant marker–trait associations were detected for these antioxidant traits. The study validated the QTLs, qANC3 and qPAC12-2 for anthocyanin content and qAC12 for ABTS activity will be useful in marker-assisted breeding. Eleven QTLs such as qTAC1.1 and qTAC5.1 controlling anthocyanin content, qSOD1.1, qSOD5.1 and qSOD10.1 for superoxide dismutase (SOD), qTFC6.1, qTFC11.1 and qTFC12.1 for total flavonoids content (TFC), qOZ8.1 and qOZ11.1 for γ-oryzanol (OZ) and qAC11.1 for ABTS activity were detected as novel loci. Chromosomal locations on 11 at 45.3 cM regulating GO, TFC and TAC, and on the chromosome 12 at 101.8 cM controlling TAC and ABTS activity, respectively, were detected as antioxidant hotspots.