Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue

Kento Ojima; Wataru Kakegawa; Tokiwa Yamasaki; Yuta Miura; Masayuki Itoh; Yukiko Michibata; Ryou Kubota; Tomohiro Doura; Eriko Miura; Hiroshi Nonaka; Seiya Mizuno; Satoru Takahashi; Michisuke Yuzaki; Itaru Hamachi; Shigeki Kiyonaka

doi:10.1038/s41467-022-30828-0

Nature Communications (Jun 2022)

Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue

Kento Ojima,
Wataru Kakegawa,
Tokiwa Yamasaki,
Yuta Miura,
Masayuki Itoh,
Yukiko Michibata,
Ryou Kubota,
Tomohiro Doura,
Eriko Miura,
Hiroshi Nonaka,
Seiya Mizuno,
Satoru Takahashi,
Michisuke Yuzaki,
Itaru Hamachi,
Shigeki Kiyonaka

Affiliations

Kento Ojima: Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University
Wataru Kakegawa: Department of Neurophysiology, Keio University School of Medicine
Tokiwa Yamasaki: Department of Neurophysiology, Keio University School of Medicine
Yuta Miura: Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University
Masayuki Itoh: Department of Neurophysiology, Keio University School of Medicine
Yukiko Michibata: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University
Ryou Kubota: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University
Tomohiro Doura: Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University
Eriko Miura: Department of Neurophysiology, Keio University School of Medicine
Hiroshi Nonaka: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University
Seiya Mizuno: Laboratory Animal Resource Center in Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba
Satoru Takahashi: Laboratory Animal Resource Center in Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba
Michisuke Yuzaki: Department of Neurophysiology, Keio University School of Medicine
Itaru Hamachi: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University
Shigeki Kiyonaka: Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University

DOI: https://doi.org/10.1038/s41467-022-30828-0
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 17

Abstract

Read online

Cell-type-specific activation of receptors is desirable for elucidating their roles in tissues or animals. Here, the authors developed a chemogenetic method for direct activation of mGlu1, a GPCR-type glutamate receptor subtype, and demonstrate its use in mouse brain tissue.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal