iScience (Jan 2024)

Proteasome assembly chaperone translation upon stress requires Ede1 phase separation at the plasma membrane

  • Thomas D. Williams,
  • Aurellia Winaya,
  • Ifeoluwapo Joshua,
  • Adrien Rousseau

Journal volume & issue
Vol. 27, no. 1
p. 108732

Abstract

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Summary: Proteome adaptation is key to cells surviving stresses. Increased translation of proteasome assembly chaperones (PACs) is critical for increasing proteasome assembly and cell degradative capacity. The endocytic protein Ede1 recruits PAC mRNA to cortical actin patches in Saccharomyces cerevisiae for translation upon stress. We show, through genetic and pharmacological studies, that this is mediated by the capacity of Ede1 to phase separate. PAC expression is maintained when we exchange the phase separating domains from Ede1 for those of unrelated proteins. Without these phase separating regions, PAC expression is not induced upon stress, preventing increased proteasome assembly, causing cell death. This work identifies a mechanism underpinning Ede1-mediated increased translation of specific mRNAs at a time when general translation is repressed.

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