Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma
Weeraya Thongkum,
Umpa Yasamut,
Koollawat Chupradit,
Supachai Sakkhachornphop,
Jiraprapa Wipasa,
Kanokporn Sornsuwan,
On-anong Juntit,
Rawiwan Pornprasit,
Wanwisa Thongkamwitoon,
Jirapan Chaichanan,
Jaruwan Khaoplab,
Chonnikarn Chanpradab,
Watchara Kasinrerk,
Chatchai Tayapiwatana
Affiliations
Weeraya Thongkum
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Umpa Yasamut
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Koollawat Chupradit
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Supachai Sakkhachornphop
Center of Biomolecular Therapy and Diagnostic, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Jiraprapa Wipasa
Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Kanokporn Sornsuwan
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
On-anong Juntit
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Rawiwan Pornprasit
Bio Innovation Building, Mahidol University, Nakhon Pathom 73170, Thailand
Wanwisa Thongkamwitoon
Bio Innovation Building, Mahidol University, Nakhon Pathom 73170, Thailand
Jirapan Chaichanan
Bio Innovation Building, Mahidol University, Nakhon Pathom 73170, Thailand
Jaruwan Khaoplab
Bio Innovation Building, Mahidol University, Nakhon Pathom 73170, Thailand
Chonnikarn Chanpradab
Bio Innovation Building, Mahidol University, Nakhon Pathom 73170, Thailand
Watchara Kasinrerk
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Chatchai Tayapiwatana
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Adult-onset immunodeficiency syndrome (AOID) patients with autoantibodies (autoAbs) against interferon-gamma (IFN-γ) generally suffer from recurrent and recalcitrant disseminated non-tuberculous mycobacterial diseases. Since the early stages of AOID do not present specific symptoms, diagnosis and treatment of the condition are not practical. A simplified diagnostic method for differentiating AOID from other immunodeficiencies, such as HIV infection, was created. Anti-IFN-γ is generally identified using enzyme-linked immunosorbent assay (ELISA), which involves an instrument and a cumbersome process. Recombinant IFN-γ indirectly conjugated to colloidal gold was used in the modified immunochromatographic (IC) strips. The biotinylated-IFN-γ was incorporated with colloidal-gold-labeled 6HIS-maltose binding protein-monomeric streptavidin (6HISMBP-mSA) and absorbed at the conjugate pad. The efficacy of the IC strip upon applying an anti-IFN-γ autoAb cut-off ELISA titer of 2500, the sensitivity and specificity were 84% and 90.24%, respectively. When a cut-off ELISA titer of 500 was applied, the sensitivity and specificity were 73.52% and 100%, respectively.
