Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape

Boyang Hua; Christopher P. Jones; Jaba Mitra; Peter J. Murray; Rebecca Rosenthal; Adrian R. Ferré-D’Amaré; Taekjip Ha

doi:10.1038/s41467-020-18283-1

Nature Communications (Sep 2020)

Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape

Boyang Hua,
Christopher P. Jones,
Jaba Mitra,
Peter J. Murray,
Rebecca Rosenthal,
Adrian R. Ferré-D’Amaré,
Taekjip Ha

Affiliations

Boyang Hua: Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine
Christopher P. Jones: Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health
Jaba Mitra: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign
Peter J. Murray: T. C. Jenkins Department of Biophysics, Johns Hopkins University
Rebecca Rosenthal: T. C. Jenkins Department of Biophysics, Johns Hopkins University
Adrian R. Ferré-D’Amaré: Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health
Taekjip Ha: Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine

DOI: https://doi.org/10.1038/s41467-020-18283-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

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Many RNAs become functional before their synthesis completes. Here the authors employ a single-molecule vectorial folding assay mimicking RNA transcription and show that the ZTP riboswitch is kinetically controlled and activated by slower unwinding and strategic pausing.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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