Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease
Joshua Millar,
Luke Gibbons,
Catia Costa,
Ella Schneider,
Johanna von Gerichten,
Melanie J. Bailey,
Susan Campbell,
Catherine Duckett,
Sarah Doyle,
Laura M. Cole
Affiliations
Joshua Millar
Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK
Luke Gibbons
Immunobiology Research Group, Department of Clinical Medicine, Trinity College Institute of Neuroscience (TCIN), School of Medicine, Trinity College Dublin (TCD), D02 R590 Dublin, Ireland
Catia Costa
Ion Beam Centre, University of Surrey, Guildford GU2 7XH, UK
Ella Schneider
Ion Beam Centre, University of Surrey, Guildford GU2 7XH, UK
Johanna von Gerichten
Department of Chemistry, University of Surrey, Guildford GU2 7XH, UK
Melanie J. Bailey
Department of Chemistry, University of Surrey, Guildford GU2 7XH, UK
Susan Campbell
Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK
Catherine Duckett
Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK
Sarah Doyle
Immunobiology Research Group, Department of Clinical Medicine, Trinity College Institute of Neuroscience (TCIN), School of Medicine, Trinity College Dublin (TCD), D02 R590 Dublin, Ireland
Laura M. Cole
Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK
The metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been utilized to describe the distribution and concentrations of essential metals in both wildtype (WT) and rhodopsin (Rho−/−) ocular tissues. The multimodal approach described is an example of complementary datasets that can be produced when employing a multifaceted analytical approach. Heterogenous distributions of copper and zinc were observable within both WT and Rho−/− tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the distributions of further trace elements notoriously problematic for ICP-MS analysis (phosphorous, Sulfur, chlorine, potassium, calcium, iron, and aluminum) were analysed by particle-induced X-ray emission (PIXE).
