Understanding the Chemical Composition and Biological Activities of Different Extracts of <i>Secamone afzelii</i> Leaves: A Potential Source of Bioactive Compounds for the Food Industry

Abstract

Secamone afzelii (Roem. & Schult.) K. Schum (family Asclepiadaceae) is a creeping woody climber used to treat ailments in many traditional medicine systems. The present study aims to examine the antioxidant and enzyme inhibition activities of S. afzelii leaf using different compositions of methanol–water mixture as an extraction solvent. The extracts were characterized by HPLC-ESI-MSn in terms of chemical compounds. The in silico results show that compound 23 (quercitrin) has the higher docking scores among the selected substances and the MD simulation revealed that the interactions with the enzymatic pocket are stable over the simulation time and strongly involve the tyrosinase catalytic Cu atoms. All together the results showed that both 80% and 100% methanolic extracts contained significantly (p p n and 6 of them were quantified using standards. Results showed that the extracts were rich in flavonoids with a relatively high abundance of two kaempferol glycosides comprising 60% of quantified compounds. The 100% and 80% methanol extracts recorded significantly (p p +++ and Cu++ was recorded from the 80% methanolic extract while 100% ethanolic extract gave the highest significant (p p S. afzelii leaf as a potential source of bioactive compounds for the food industry, for the preparation of food supplements and functional foods.

Keywords

• <i>Secamone afzelii</i>
• antioxidant
• enzyme inhibition
• chemical profile
• extraction solvent
• functional food