Detection of Cu<sup>2+</sup> Ions with GGH Peptide Realized with Si-Nanoribbon ISFET
Olena Synhaivska,
Yves Mermoud,
Masoud Baghernejad,
Israel Alshanski,
Mattan Hurevich,
Shlomo Yitzchaik,
Mathias Wipf,
Michel Calame
Affiliations
Olena Synhaivska
Transport at Nanoscale Interfaces Laboratory, Empa–Swiss Federal Laboratories for Materials Science and Technology, Ueberlandstrasse 129, CH-8600 Duebendorf, Switzerland
Yves Mermoud
Transport at Nanoscale Interfaces Laboratory, Empa–Swiss Federal Laboratories for Materials Science and Technology, Ueberlandstrasse 129, CH-8600 Duebendorf, Switzerland
Masoud Baghernejad
Transport at Nanoscale Interfaces Laboratory, Empa–Swiss Federal Laboratories for Materials Science and Technology, Ueberlandstrasse 129, CH-8600 Duebendorf, Switzerland
Israel Alshanski
Institute of Chemistry, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
Mattan Hurevich
Institute of Chemistry, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
Shlomo Yitzchaik
Institute of Chemistry, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
Mathias Wipf
Transport at Nanoscale Interfaces Laboratory, Empa–Swiss Federal Laboratories for Materials Science and Technology, Ueberlandstrasse 129, CH-8600 Duebendorf, Switzerland
Michel Calame
Transport at Nanoscale Interfaces Laboratory, Empa–Swiss Federal Laboratories for Materials Science and Technology, Ueberlandstrasse 129, CH-8600 Duebendorf, Switzerland
The presence of heavy metal ions such as copper in the human body at certain concentrations and specific conditions can lead to the development of different diseases. The currently available analytical detection methods remain expensive, time-consuming, and often require sample pre-treatment. The development of specific and quantitative, easy-in-operation, and cost-effective devices, capable of monitoring the level of Cu2+ ions in environmental and physiological media, is necessary. We use silicon nanoribbon (SiNR) ion-sensitive field effect transistor (ISFET) devices modified with a Gly−Gly−His peptide for the detection of copper ions in a large concentration range. The specific binding of copper ions causes a conformational change of the ligand, and a deprotonation of secondary amine groups. By performing differential measurements, we gain a deeper insight into the details of the ion−ligand interaction. We highlight in particular the importance of considering non-specific interactions to explain the sensors’ response.
