Endophytic bacteria isolated from higher plant in Aceh, Indonesia, and their chemical compounds activity against Fusarium oxysporum f. sp. lycopersici

Abstract

Abstract Background Endophytic bacteria are an association between bacteria and plant tissue that could play a role as a biocontrol agent. Main body Endophytic bacteria were isolated from several high root plants in Aceh, Indonesia. This study aimed to detect the chemical compounds of the potential endophytic bacteria as a biocontrol agent against Fusarium oxysporum f. sp. lycopersici (FOL). There were 198 endophytic bacterial isolates detected in roots of 9 higher plant. The hypersensitive reaction showed that 193 isolated endophytic bacteria were non-pathogenic. There were 13 isolated endophytic bacteria that worked to inhibit FOL between 50.0 and 89.2%; such endophytic bacteria were isolated from Solanum lycopersicum L., Psidium guajava L., Dendrocalamus asper (Schult with f.) Backer ex Heyne, Pinus merkusii L., Theobroma cacao L., and Albizia chinensis L. Molecular identification using 16S rRNA gene sequence confirmed that the endophytic bacteria were derived from species Pseudomonas aeruginosa, P. mosselii, Arthrobacter sp., Bacillus cereus, B. thuringiensis, and Serratia marcescens. P. aeruginosa that showed the highest inhibition was analyzed using GC-MS analysis. The analysis identified that antibiotics as Pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- was produced by P. aeruginosa succeeded in suppressing FOL. Conclusion The study recommends the species P. aeruginosa, as effective endophytic bacteria for the control of FOL pathogen.

Keywords

• Endophytic bacteria
• Dual culture
• Fusarium oxysporum f. sp. lycopersici
• Antibiotics
• Molecular identification
• GC-MS analysis