A simple lipase assay using trichloroacetic acid

Michael C. Schotz; Arlene S. Garfinkel

Journal of Lipid Research (Nov 1972)

A simple lipase assay using trichloroacetic acid

Michael C. Schotz,
Arlene S. Garfinkel

Affiliations

Michael C. Schotz: Research, Veterans Administration, Wadsworth Hospital Center, Los Angeles, California 90073, and; Department of Medicine, UCLA Center for the Health Sciences, Los Angeles, California 90024
Arlene S. Garfinkel: Research, Veterans Administration, Wadsworth Hospital Center, Los Angeles, California 90073, and; Department of Medicine, UCLA Center for the Health Sciences, Los Angeles, California 90024

Journal volume & issue: Vol. 13, no. 6
pp. 824 – 826

Abstract

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An extremely rapid and sensitive assay for lipoprotein lipase activity, suitable for routine determinations, is described. The substrate for the assay is emulsified [2-3H]glyceryl trioleate, activated by serum. The method is based on trichloroacetic acid precipitation of unreacted substrate and measurement of 3H-labeled glycerol.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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Abstract

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