Photoprotection and skin irritation effect of hydrogels containing hydroalcoholic extract of red propolis: A natural pathway against skin cancer
Cinthia M. Batista,
Luma A. de Queiroz,
Ângela V.F. Alves,
Elisiane C.A. Reis,
Fagne A. Santos,
Tailaine N. Castro,
Bruno S. Lima,
Adriano N.S. Araújo,
Charles A.P. Godoy,
Patricia Severino,
Amanda Cano,
Antonello Santini,
Raffaele Capasso,
Ricardo L.C. de Albuquerque Júnior,
Juliana C. Cardoso,
Eliana B. Souto
Affiliations
Cinthia M. Batista
Biotechnological Postgraduate Program, University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil
Luma A. de Queiroz
Biotechnological Postgraduate Program, University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil
Ângela V.F. Alves
Biotechnological Postgraduate Program, University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil
Elisiane C.A. Reis
Institute of Technology and Research (ITP), University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil
Fagne A. Santos
Biotechnological Postgraduate Program, University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil
Tailaine N. Castro
Biotechnological Postgraduate Program, University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil
Bruno S. Lima
Department of Pharmacy, Federal University of Sergipe, Aracaju, Sergipe, Brazil
Adriano N.S. Araújo
Department of Pharmacy, Federal University of Sergipe, Aracaju, Sergipe, Brazil
Charles A.P. Godoy
Biotechnological Postgraduate Program, University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil
Patricia Severino
Biotechnological Postgraduate Program, University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil; Institute of Technology and Research (ITP), University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil
Amanda Cano
Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, 08028, Barcelona, Spain; Institute of Nanoscience and Nanotechnology, Universitat de Barcelona, 08028, Barcelona, Spain
Antonello Santini
Department of Pharmacy, University of Napoli Federico II, 80131, Napoli, Italy
Raffaele Capasso
Department of Agricultural Sciences, University of Naples Federico II, 80055, Portici, Naples, Italy
Ricardo L.C. de Albuquerque Júnior
Biotechnological Postgraduate Program, University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil; Institute of Technology and Research (ITP), University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil
Juliana C. Cardoso
Biotechnological Postgraduate Program, University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil; Institute of Technology and Research (ITP), University of Tiradentes (Unit), Av. Murilo Dantas 300, 49010-390, Aracaju, Brazil; Corresponding author.
Eliana B. Souto
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; CEB—Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; LABBELS—Associate Laboratory, Braga, Guimarães, Portugal; Corresponding author.
The use of natural products in sunscreen formulations as a prophylactic measure against skin cancer is receiving special attention attributed to the photoprotective and antioxidant properties of their chemical components. In this work, we describe the development of topical hydrogel formulations containing hydroalcoholic extract of red propolis (HERP), and the evaluation of the dermal sensitizing effect of the developed products. Sunscreen formulations composed of HERP in different concentrations (1.5, 2.5 or 3.5% w/w) alone or in combination with a chemical (octyl methoxycinnamate) and/or physical (titanium dioxide) filters were developed using poloxamer 407 as gel basis. The preliminary and accelerated stability tests, texture analysis and spreadability tests were performed. All formulations revealed to be stable in preliminary stability assessment. The formulations containing HERP 1.5 and 2.5% alone or associated with the filters showed intense modifications during accelerated stability test, which were confirmed by rheological analyses. The incorporation of HERP and filters in the poloxamer hydrogel decreased the toughness of product (p < 0.05) and the formulation containing HERP alone presented the lowest adhesivity (p < 0.001). The incorporation of HERP in the hydrogel decreased the poloxamer transition temperature, showing different rheological behavior with the increase of HERP concentration. The developed formulations were stable, exhibited non-Newtonian and pseudoplastic behavior, showing in vivo skin compatibility and no skin irritancy.
