Carcinogenic effects of N-nitroso-3-(substituted phenylimino)-indolin-2-one derivatives

Abstract

Aim: A novel series of N-nitroso-3-(substituted phenylimino)-indolin-2-one 3a-h was synthesized and tested for carcinogenic effects. Materials and Methods: The synthesized pyrazole derivatives′ chemical structures were proved by means of their infra red (IR), proton nuclear magnetic resonance ( 1 H-NMR), and mass,and confirmed by elemental analyses. The carcinogenic activity was assessed by 3-(4,5dimethyl thiazole-2yl)-2,5-diphenyltetrazoliumbromide (MTT) cell-viability assay. Results: The results show that most of the synthesized compounds exhibit significant carcinogenic activities. Among the synthesized compounds, N-nitroso-3-(2,4-dinitrophenylimino)-indolin-2-one 3h exhibited the most potent carcinogenic activity. Conclusion: The structure-activity relationship (SAR) studies show that the nature as well as the position of the amine are important for deciding the activity profile of the indolin-2-one derivatives, which reiterates the need for further experimental investigations.

Keywords

• Carcinogenic activity
• MTT cell-viability assay
• N-nitrosoisatin
• Schiff base