Biogenic Synthesis of NiO Nanoparticles Using <i>Areca catechu</i> Leaf Extract and Their Antidiabetic and Cytotoxic Effects
Shwetha U R,
Rajith Kumar C R,
Kiran M S,
Virupaxappa S. Betageri,
Latha M S,
Ravindra Veerapur,
Ghada Lamraoui,
Abdulaziz A. Al-Kheraif,
Abdallah M. Elgorban,
Asad Syed,
Chandan Shivamallu,
Shiva Prasad Kollur
Affiliations
Shwetha U R
Research Centre, Department of Chemistry, GM Institute of Technology, Davangere 577 006, Karnataka, India
Rajith Kumar C R
Research Centre, Department of Chemistry, GM Institute of Technology, Davangere 577 006, Karnataka, India
Kiran M S
Research Centre, Department of Chemistry, GM Institute of Technology, Davangere 577 006, Karnataka, India
Virupaxappa S. Betageri
Research Centre, Department of Chemistry, GM Institute of Technology, Davangere 577 006, Karnataka, India
Latha M S
Department of Chemistry, R L Science Institute, Belagavi 590 001, Karnataka, India
Ravindra Veerapur
Department of Metallurgy and Materials Engineering, Malawi Institute of Technology, Malawi University of Science and Technology, Limbe P.O. Box 5916, Malawi
Ghada Lamraoui
Faculty of Nature and Life Sciences, Earth and Universe Sciences, University of Tlemcen, Tlemcen 13000, Algeria
Abdulaziz A. Al-Kheraif
Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia
Abdallah M. Elgorban
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Asad Syed
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Chandan Shivamallu
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru 570 015, Karnataka, India
Shiva Prasad Kollur
Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru 570 026, Karnataka, India
Nanoworld is an attractive sphere with the potential to explore novel nanomaterials with valuable applications in medicinal science. Herein, we report an efficient and ecofriendly approach for the synthesis of Nickel oxide nanoparticles (NiO NPs) via a solution combustion method using Areca catechu leaf extract. As-prepared NiO NPs were characterized using various analytical tools such as powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and UV-Visible spectroscopy (UV-Vis). XRD analysis illustrates that synthesized NiO NPs are hexagonal structured crystallites with an average size of 5.46 nm and a hexagonal-shaped morphology with slight agglomeration. The morphology, size, and shape of the obtained material was further confirmed using SEM and TEM analysis. In addition, as-prepared NiO NPs have shown potential antidiabetic and anticancer properties. Our results suggest that the inhibition of α-amylase enzyme with IC 50 value 268.13 µg/mL may be one of the feasible ways through which the NiO NPs exert their hypoglycemic effect. Furthermore, cytotoxic activity performed using NiO NPs exhibited against human lung cancer cell line (A549) proved that the prepared NiO NPs have significant anticancer activity with 93.349 μg/mL at 50% inhibition concentration. The biological assay results revealed that NiO NPs exhibited significant cytotoxicity against human lung cancer cell line (A549) in a dose-dependent manner from 0–100 μg/mL, showing considerable cell viability. Further, the systematic approach deliberates the NiO NPs as a function of phenolic extracts of A. catechu with vast potential for many biological and biomedical applications.
