The impact of Allgower-Donati suture pattern and postoperative sweet foods on wound suture breakage in experimental rats
Weifeng Li,
Feng Xiong,
Cheng Yao,
Tingbao Zhang,
Liangshuang Zhou,
Zhanyue Zhang,
Zhaodong Wang,
Yingji Mao,
Pinghui Zhou,
Jianzhong Guan
Affiliations
Weifeng Li
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; First Department of Orthopedics, People's Hospital of Lixin County, Bozhou, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, China
Feng Xiong
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China
Cheng Yao
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China
Tingbao Zhang
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China
Liangshuang Zhou
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China
Zhanyue Zhang
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, China
Zhaodong Wang
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, China
Yingji Mao
Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, China
Pinghui Zhou
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, China
Jianzhong Guan
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China; Anhui Province Key Laboratory of Tissue Transplantation, Bengbu, China; Corresponding author. Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu, China.
Background: Wound gnawing and/or scratching in rats often occurs in experimental models, causing suture breakage and wound dehiscence, and consequently affecting experimental results and wasting resources. This study aimed to investigate the impact of the combined postoperative use of the Allgower-Donati (A-D) suture pattern and sweet foods on suture breakage, inflammation, and healing in wounds. Materials and methods: Sprague Dawley (SD) rats (n = 48) were treated for linear wounds on the back by four procedures: simple suture, simple suture with postoperative sweet foods, A-D suture, and A-D suture with postoperative sweet foods. Additionally, CD68 immunofluorescence and CD31 immunohistochemistry were used to analyze wound inflammation and vascularization, respectively, on postoperative day 7. Sirius red staining was used to assess collagen deposition on postoperative day 14. Results: Gnawing and scratching of wound sutures were significantly reduced in treated rats (P < 0.01). Neovascularization and collagen deposition were significantly increased (P < 0.001), and inflammatory responses were significantly reduced (P < 0.001) in animals receiving AD sutures and postoperative sweet foods. CD31/CD68 analyses showed that A-D suture and postoperative sweet foods regulated wound angiogenesis and attenuated wound inflammation. Conclusions: Sweet food provision after A-D suture union surgery could reduce wound gnawing and/or scratching, suture breakage, incisional dehiscence, wound inflammation, and promote wound healing in rats.
