Viruses (Jan 2024)

Genomic Characterization of a Wild-Type <i>Bovine alphaherpesvirus 1</i> (BoAHV-1) Strain Isolated in an Outbreak in Central Italy

  • Stefano Petrini,
  • Valentina Curini,
  • Cecilia Righi,
  • Cesare Cammà,
  • Valeria Di Lollo,
  • Elena Tinelli,
  • Luana Fiorella Mincarelli,
  • Elisabetta Rossi,
  • Giulia Costantino,
  • Barbara Secondini,
  • Silvia Pirani,
  • Monica Giammarioli,
  • Francesco Feliziani

DOI
https://doi.org/10.3390/v16010150
Journal volume & issue
Vol. 16, no. 1
p. 150

Abstract

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Bovine alphaherpesvirus-1 (BoAHV-1) infection is common in cattle worldwide. However, information on the spread of BoAHV-1-circulating strains in Italy remains limited. In this study, we investigated an outbreak characterized by severe respiratory symptoms in a cattle herd (n = 30) located in Central Italy. BoAHV-1 was isolated from three cattle in a cell culture, which confirmed viral infection. Next, we characterized one (16453/07 TN) of the three isolates of BoAHV-1 using whole-genome sequencing. BLASTn and phylogenetic analysis revealed a nucleotide identity >99% with all BoAHV-1 strains belonging to subtype 1.1, highlighting the genetic stability of the virus. This study reports the first full genomic characterization of a BoAHV-1 isolate in Italy, enriching our understanding of the genetic characteristics of the circulating BoAHV-1 strain in Italy.

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