Preparation of Protein Aerogel Particles for the Development of Innovative Drug Delivery Systems
Daria Lovskaya,
Anna Bezchasnyuk,
Maria Mochalova,
Pavel Tsygankov,
Artem Lebedev,
Yana Zorkina,
Eugene Zubkov,
Aleksandra Ochneva,
Olga Gurina,
Artemiy Silantyev,
Alexander Majouga,
Natalia Menshutina
Affiliations
Daria Lovskaya
Department of Chemical and Pharmaceutical Engineering, Mendeleev University of Chemical Technology, 125047 Moscow, Russia
Anna Bezchasnyuk
Department of Chemical and Pharmaceutical Engineering, Mendeleev University of Chemical Technology, 125047 Moscow, Russia
Maria Mochalova
Department of Chemical and Pharmaceutical Engineering, Mendeleev University of Chemical Technology, 125047 Moscow, Russia
Pavel Tsygankov
Department of Chemical and Pharmaceutical Engineering, Mendeleev University of Chemical Technology, 125047 Moscow, Russia
Artem Lebedev
Department of Chemical and Pharmaceutical Engineering, Mendeleev University of Chemical Technology, 125047 Moscow, Russia
Yana Zorkina
Department of Basic and Applied Neurobiology, V. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Kropotkinsky Per. 23, 119034 Moscow, Russia
Eugene Zubkov
Department of Basic and Applied Neurobiology, V. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Kropotkinsky Per. 23, 119034 Moscow, Russia
Aleksandra Ochneva
Department of Basic and Applied Neurobiology, V. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Kropotkinsky Per. 23, 119034 Moscow, Russia
Olga Gurina
Department of Basic and Applied Neurobiology, V. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Kropotkinsky Per. 23, 119034 Moscow, Russia
Artemiy Silantyev
Department of Basic and Applied Neurobiology, V. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Kropotkinsky Per. 23, 119034 Moscow, Russia
Alexander Majouga
Department of Chemical and Pharmaceutical Engineering, Mendeleev University of Chemical Technology, 125047 Moscow, Russia
Natalia Menshutina
Department of Chemical and Pharmaceutical Engineering, Mendeleev University of Chemical Technology, 125047 Moscow, Russia
The research was oriented towards the preparation of aerogel particles based on egg white and whey protein isolate using various dispersion methods: dripping, spraying, and homogenization. Based on the results of analytical studies, the most appropriate samples were selected to obtain aerogels loaded with the drug. The results of the experimental research were used to study methods for obtaining nasal drug delivery systems based on aerogels. Protein aerogels were obtained by thermal gelation followed by supercritical drying. The obtained particles of protein aerogels have a specific surface area of up to 350 m2/g with a pore volume of up to 2.9 cm3/g, as well as a porosity of up to 95%. The results of experimental studies have shown that changing the dispersion method makes it possible to control the structural characteristics of protein aerogel particles. The results of the studies were applied to obtain innovative nasal drug delivery systems for the treatment of socially significant diseases. Analytical studies were conducted to determine the amount and state of adsorbed drugs in protein aerogel particles, as well as in vivo experiments on the distribution of clomipramine in blood plasma and brain tissue of rats to study the pharmacokinetics and bioavailability of the resulting drug-loaded protein aerogel.
