Proapoptotic properties of total phytohemagglutinine and its individual isolectins in human cell culture 4BL

Ukrainian Biochemical Journal. 2014;86(4):103-109 DOI 10.15407/ubj86.04.103

 

Journal Homepage

Journal Title: Ukrainian Biochemical Journal

ISSN: 2409-4943 (Print); 2413-5003 (Online)

Publisher: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.

Society/Institution: Palladin institute of Biochemistry of the NASU

LCC Subject Category: Science: Chemistry: Organic chemistry: Biochemistry

Country of publisher: Ukraine

Language of fulltext: English

Full-text formats available: PDF

 

AUTHORS

T. O. Kochubei (Institute of Molecular Biology and Genetics, National Аcademy of Sciences of Ukraine, Кyiv;)
O. V. Maksymchuk (Institute of Molecular Biology and Genetics, National Аcademy of Sciences of Ukraine, Кyiv;)
L. L. Маcewicz (Institute of Molecular Biology and Genetics, National Аcademy of Sciences of Ukraine, Кyiv;)
О. O. Piven (Institute of Molecular Biology and Genetics, National Аcademy of Sciences of Ukraine, Кyiv;)
L. L. Lukash (Institute of Molecular Biology and Genetics, National Аcademy of Sciences of Ukraine, Кyiv;)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 16 weeks

 

Abstract | Full Text

Phytohemagglutinine (PHA) is widely investigated lectin with mitogenic properties. Recently it was shown that PHA is not only cell proliferation inducer, but also has a toxic or cytostatic effect. However concentration dependence and molecular mecha­nisms of this effect are not enough investigated­. To study proapoptotic properties of total phytohemagglutinine and its isolectins in human cell culture of not tumor origin 4BL we observed a change in the frequency of apoptotic cells in the tested cell culture under the influence of the total phytohemagglutinine and erythroagglutinin by the method of specific color luminescent dye. The activation of caspases-3 and -8 and induction of protein Bax expression under the influence of lectins were detected by Western blot analysis. It was revealed that erythroagglutinin induced apoptosis with the highest efficiency compared with leukoagglutinin and total phytohemagglutinine. The induction of apoptosis in human cell culture of not tumor origin 4BL is probably caused by activating caspase-dependent and mitochondrial signalling.