Microsatellite Markers in <i>Rhodiola</i> (Crassulaceae), a Medicinal Herb Genus Widely Used in Traditional Chinese Medicine

Jianling You; Wensheng Liu; Yao Zhao; Yongqing Zhu; Wenju Zhang; Yuguo Wang; Fan Lu; Zhiping Song

doi:10.3732/apps.1200219

Applications in Plant Sciences (Mar 2013)

Microsatellite Markers in <i>Rhodiola</i> (Crassulaceae), a Medicinal Herb Genus Widely Used in Traditional Chinese Medicine

Jianling You,
Wensheng Liu,
Yao Zhao,
Yongqing Zhu,
Wenju Zhang,
Yuguo Wang,
Fan Lu,
Zhiping Song

Affiliations

Jianling You: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, People's Republic of China
Wensheng Liu: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, People's Republic of China
Yao Zhao: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, People's Republic of China
Yongqing Zhu: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, People's Republic of China
Wenju Zhang: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, People's Republic of China
Yuguo Wang: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, People's Republic of China
Fan Lu: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, People's Republic of China
Zhiping Song: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, People's Republic of China

DOI: https://doi.org/10.3732/apps.1200219
Journal volume & issue: Vol. 1, no. 3
p. 1200219

Abstract

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Premise of the study: Microsatellite loci are described for Rhodiola, a medicinal herb genus widely used in traditional Chinese medicine. Methods and Results: A total of 17 polymorphic microsatellite primer pairs were developed using the combined biotin-capture method. The number of alleles per locus ranged from one to 12 across 192 individuals from R. bupleuroides, R. crenulata, R. fastigiata, and R. sacra, and the mean observed and expected heterozygosities ranged from 0.177 to 0.412 and from 0.363 to 0.578, respectively. Conclusions: The results demonstrate the potential use of this new set of microsatellite markers for genotyping individuals and estimating genetic diversity in Rhodiola.

Published in Applications in Plant Sciences

ISSN: 2168-0450 (Online)
Publisher: Wiley
Country of publisher: United States
LCC subjects: Science: Biology (General); Science: Botany
Website: https://bsapubs.onlinelibrary.wiley.com/journal/21680450

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