Communications Biology (Jan 2022)
Cooperative allostery and structural dynamics of streptavidin at cryogenic- and ambient-temperature
- Esra Ayan,
- Busra Yuksel,
- Ebru Destan,
- Fatma Betul Ertem,
- Gunseli Yildirim,
- Meryem Eren,
- Oleksandr M. Yefanov,
- Anton Barty,
- Alexandra Tolstikova,
- Gihan K. Ketawala,
- Sabine Botha,
- E. Han Dao,
- Brandon Hayes,
- Mengning Liang,
- Matthew H. Seaberg,
- Mark S. Hunter,
- Alexander Batyuk,
- Valerio Mariani,
- Zhen Su,
- Frederic Poitevin,
- Chun Hong Yoon,
- Christopher Kupitz,
- Aina Cohen,
- Tzanko Doukov,
- Raymond G. Sierra,
- Çağdaş Dağ,
- Hasan DeMirci
Affiliations
- Esra Ayan
- Department of Molecular Biology and Genetics, Koc University
- Busra Yuksel
- Department of Molecular Biology and Genetics, Koc University
- Ebru Destan
- Department of Molecular Biology and Genetics, Koc University
- Fatma Betul Ertem
- Department of Molecular Biology and Genetics, Koc University
- Gunseli Yildirim
- Department of Molecular Biology and Genetics, Koc University
- Meryem Eren
- Department of Molecular Biology and Genetics, Koc University
- Oleksandr M. Yefanov
- Deutsches Elektronen-Synchrotron
- Anton Barty
- Deutsches Elektronen-Synchrotron
- Alexandra Tolstikova
- Deutsches Elektronen-Synchrotron
- Gihan K. Ketawala
- Department of Physics, Arizona State University
- Sabine Botha
- Department of Physics, Arizona State University
- E. Han Dao
- Stanford PULSE Institute, SLAC National Laboratory
- Brandon Hayes
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Mengning Liang
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Matthew H. Seaberg
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Mark S. Hunter
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Alexander Batyuk
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Valerio Mariani
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Zhen Su
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Frederic Poitevin
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Chun Hong Yoon
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Christopher Kupitz
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Aina Cohen
- Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory
- Tzanko Doukov
- Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory
- Raymond G. Sierra
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Çağdaş Dağ
- Department of Molecular Biology and Genetics, Koc University
- Hasan DeMirci
- Department of Molecular Biology and Genetics, Koc University
- DOI
- https://doi.org/10.1038/s42003-021-02903-7
- Journal volume & issue
-
Vol. 5,
no. 1
pp. 1 – 13
Abstract
Ayan et al. report two structures of the protein streptavidin - one at ambient temperature determined using serial femtosecond crystallography and a second one determined at cryogenic temperature. These results provide insights into the structural dynamics of apo streptavidin and reveal a cooperative allostery between monomers for binding to biotin, and the findings are supported by GNM analysis.
