Use of Urine in the Nutrition of Chlorella Vulgaris

Abstract

We have studied the effect of increasing concentrations of human native urine, NaCl, urea and NH4Cl on the batch culture of Chorella vulgaris. It was found that the concentration threshold for the culture growth were addition of 50 ml/l of urine, 30 g/l of NaCl, 800 mg/l of urea, and 20 g/l of NH4Cl to the medium. A culture medium for Chlorella was suggested containing the acid extract of urine ash and native urine. Several ways of nitrogen addition to the experimental solution of Chlorella were considered: HNO3, NH4Cl, urea and native urine. The optimal ratios of urine ash water extract, native urine and HNO3 in the medium solution were determined to maintain Chlorella growth. Comparative data were given on the biochemical values of the biomass of the C. vulgaris batch and continuous cultures according to the following parameters: mineral, amino acid and fatty acid contents, proteins, lipids, carbohydrates and vitamins. The values were also compared to those in cultures of cyanobacteria Spirullina platensis and Oscilatoria deflexa grown in the batch mode.

Keywords

• urine
• Chorella; nutrition
• dry mineralization