Nature Communications (Feb 2021)
PRMT5 inhibition disrupts splicing and stemness in glioblastoma
- Patty Sachamitr,
- Jolene C. Ho,
- Felipe E. Ciamponi,
- Wail Ba-Alawi,
- Fiona J. Coutinho,
- Paul Guilhamon,
- Michelle M. Kushida,
- Florence M. G. Cavalli,
- Lilian Lee,
- Naghmeh Rastegar,
- Victoria Vu,
- María Sánchez-Osuna,
- Jasmin Coulombe-Huntington,
- Evgeny Kanshin,
- Heather Whetstone,
- Mathieu Durand,
- Philippe Thibault,
- Kirsten Hart,
- Maria Mangos,
- Joseph Veyhl,
- Wenjun Chen,
- Nhat Tran,
- Bang-Chi Duong,
- Ahmed M. Aman,
- Xinghui Che,
- Xiaoyang Lan,
- Owen Whitley,
- Olga Zaslaver,
- Dalia Barsyte-Lovejoy,
- Laura M. Richards,
- Ian Restall,
- Amy Caudy,
- Hannes L. Röst,
- Zahid Quyoom Bonday,
- Mark Bernstein,
- Sunit Das,
- Michael D. Cusimano,
- Julian Spears,
- Gary D. Bader,
- Trevor J. Pugh,
- Mike Tyers,
- Mathieu Lupien,
- Benjamin Haibe-Kains,
- H. Artee Luchman,
- Samuel Weiss,
- Katlin B. Massirer,
- Panagiotis Prinos,
- Cheryl H. Arrowsmith,
- Peter B. Dirks
Affiliations
- Patty Sachamitr
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Jolene C. Ho
- Structural Genomics Consortium, University of Toronto
- Felipe E. Ciamponi
- Center for Molecular Biology and Genetic Engineering, University of Campinas (UNICAMP)
- Wail Ba-Alawi
- Princess Margaret Cancer Centre, University Health Network
- Fiona J. Coutinho
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Paul Guilhamon
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Michelle M. Kushida
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Florence M. G. Cavalli
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Lilian Lee
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Naghmeh Rastegar
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Victoria Vu
- Structural Genomics Consortium, University of Toronto
- María Sánchez-Osuna
- Institute for Research in Immunology and Cancer, Université de Montréal
- Jasmin Coulombe-Huntington
- Institute for Research in Immunology and Cancer, Université de Montréal
- Evgeny Kanshin
- Institute for Research in Immunology and Cancer, Université de Montréal
- Heather Whetstone
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Mathieu Durand
- RNomics Platform, Université de Sherbrooke
- Philippe Thibault
- RNomics Platform, Université de Sherbrooke
- Kirsten Hart
- Structural Genomics Consortium, University of Toronto
- Maria Mangos
- Structural Genomics Consortium, University of Toronto
- Joseph Veyhl
- Structural Genomics Consortium, University of Toronto
- Wenjun Chen
- Structural Genomics Consortium, University of Toronto
- Nhat Tran
- Structural Genomics Consortium, University of Toronto
- Bang-Chi Duong
- Structural Genomics Consortium, University of Toronto
- Ahmed M. Aman
- Ontario Institute for Cancer Research
- Xinghui Che
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Xiaoyang Lan
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- Owen Whitley
- The Donnelly Centre, University of Toronto
- Olga Zaslaver
- The Donnelly Centre, University of Toronto
- Dalia Barsyte-Lovejoy
- Structural Genomics Consortium, University of Toronto
- Laura M. Richards
- Princess Margaret Cancer Centre, University Health Network
- Ian Restall
- Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary
- Amy Caudy
- The Donnelly Centre, University of Toronto
- Hannes L. Röst
- The Donnelly Centre, University of Toronto
- Zahid Quyoom Bonday
- Lilly Research Laboratories, Eli Lilly and Company
- Mark Bernstein
- Division of Neurosurgery, Department of Surgery, University of Toronto
- Sunit Das
- Division of Neurosurgery, Department of Surgery, University of Toronto
- Michael D. Cusimano
- Division of Neurosurgery, Department of Surgery, St. Michael’s Hospital
- Julian Spears
- Division of Neurosurgery, Department of Surgery, University of Toronto
- Gary D. Bader
- The Donnelly Centre, University of Toronto
- Trevor J. Pugh
- Princess Margaret Cancer Centre, University Health Network
- Mike Tyers
- Institute for Research in Immunology and Cancer, Université de Montréal
- Mathieu Lupien
- Princess Margaret Cancer Centre, University Health Network
- Benjamin Haibe-Kains
- Princess Margaret Cancer Centre, University Health Network
- H. Artee Luchman
- Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary
- Samuel Weiss
- Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary
- Katlin B. Massirer
- Center for Molecular Biology and Genetic Engineering, University of Campinas (UNICAMP)
- Panagiotis Prinos
- Structural Genomics Consortium, University of Toronto
- Cheryl H. Arrowsmith
- Structural Genomics Consortium, University of Toronto
- Peter B. Dirks
- Developmental and Stem Cell Biology Program and Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
- DOI
- https://doi.org/10.1038/s41467-021-21204-5
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 17
Abstract
The arginine methyltransferase PRMT5 is over-expressed in cancer and has a role in the maintenance of stem cells. Here, the authors show that PRMT5 inhibitors can block the growth of patient derived glioblastoma stem cell cultures in vitro and in vivo, suggesting that PRMT5 inhibition may be a useful therapeutic strategy
