Data on the putative role of p53 in breast cancer cell adhesion: Technical information for adhesion assay
Kallirroi Voudouri,
Dragana Nikitovic,
Aikaterini Berdiaki,
John Tsiaoussis,
Dimitris Kletsas,
Nikos K. Karamanos,
George N. Tzanakakis
Affiliations
Kallirroi Voudouri
Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece
Dragana Nikitovic
Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece
Aikaterini Berdiaki
Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece
John Tsiaoussis
Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece
Dimitris Kletsas
Laboratory of Cell Proliferation and Ageing, Institute of Biology, National Center of Scientific Research "Demokritos", Athens, Greece
Nikos K. Karamanos
Biochemistry, Biochemical Analysis and Matrix Pathobiology Res. Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26110 Patras, Greece
George N. Tzanakakis
Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece
In this data article, the potential role of p53 tumor suppressor gene (p53) on the attachment ability of MCF-7 breast cancer cells was investigated. In our main article, “IGF-I/ EGF and E2 signaling crosstalk through IGF-IR conduit point affect breast cancer cell adhesion” (K. Voudouri, D. Nikitovic, A. Berdiaki, D. Kletsas, N.K. Karamanos, G.N. Tzanakakis, 2016) [1], we describe the key role of IGF-IR in breast cancer cell adhesion onto fibronectin (FN). p53 tumor suppressor gene is a principal regulator of cancer cell proliferation. Various data have demonstrated an association between p53 and IGF-IR actions on cell growth through its’ putative regulation of IGF-IR expression. According to our performed experiments, p53 does not modify IGF-IR expression and does not affect basal MCF-7 cells adhesion onto FN. Moreover, technical details about the performance of adhesion assay onto the FN substrate were provided.
