Biomedical and Biotechnology Research Journal (Jan 2023)

Angiogenic potential of plasma-derived extracellular vesicles from impaired fasting glucose patients: A pilot study

  • Rocio Castillo-Sanchez,
  • Maria Candia-Plata,
  • Astrid Ramirez-Romero,
  • Ana Mata-Pineda,
  • Juan Martinez-Soto,
  • Luis Lopez-Soto,
  • Jose Galvan-Moroyoqui,
  • Ramon Iniguez Palomares,
  • Cesar Rodriguez-Beas,
  • Mario Alvarez-Ramos,
  • Eduardo Perez-Salazar,
  • Adriana Soto-Guzman

DOI
https://doi.org/10.4103/bbrj.bbrj_56_23
Journal volume & issue
Vol. 7, no. 2
pp. 187 – 194

Abstract

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Background: The goal of this study was to analyze the in vitro effect of plasma-isolated extracellular vesicles (EVs) from patients with impaired fasting glucose (IFG) on cell migration and angiogenic score on human endothelial cells (EA.hy926) cultures. Methods: Plasma samples from five patients with IFG, five with Type 2 diabetes mellitus (T2DM), and five normoglycemic subjects (controls) were used. Plasma-derived EVs were characterized by electron microscopy, dynamic light scattering and Western blotting, vascular endothelial growth factor receptor 2 (VEGFR-2), and endoglin detected in EVs by flow cytometry; wound closure assays and angiogenic score by matrigel assays in EA.hy926 cells were performed. Results: EA.hy926 cell migration induced by plasma-derived EVs from patients with IFG was greater than in control subjects (P = 0.023). EVs from patients with T2DM and IFG induced higher angiogenic scores than EVs from control subjects (P = 0.012 and P = 0.036, respectively). Conclusions: Endoglin and VEGFR-2 levels in EVs from IFG or T2DM patients were not different from those in control subjects. Plasma-derived EVs from patients with IFG and T2DM positively influenced human endothelial cell migration and angiogenic activity in vitro.

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