Establishment of TUSMi009-A, an induced pluripotent stem cell (iPSC) line from a 24-year-old Chinese Han patient with gliocytoma

Kui Chen; Zhongwei Zhuang; Qi Wang; Keqin Li; Siyi Xu; Min Liu; Kuiming Zhang; Liang Wei; Yanfei Zhang; Hao Lian; Bei Zhang; Chunlong Zhong

Stem Cell Research (Oct 2021)

Establishment of TUSMi009-A, an induced pluripotent stem cell (iPSC) line from a 24-year-old Chinese Han patient with gliocytoma

Kui Chen,
Zhongwei Zhuang,
Qi Wang,
Keqin Li,
Siyi Xu,
Min Liu,
Kuiming Zhang,
Liang Wei,
Yanfei Zhang,
Hao Lian,
Bei Zhang,
Chunlong Zhong

Affiliations

Kui Chen: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China
Zhongwei Zhuang: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China
Qi Wang: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China
Keqin Li: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China
Siyi Xu: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China
Min Liu: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China
Kuiming Zhang: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China
Liang Wei: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China
Yanfei Zhang: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China
Hao Lian: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Corresponding authors.
Bei Zhang: Department of Neurology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Corresponding authors.
Chunlong Zhong: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Corresponding authors.

Journal volume & issue: Vol. 56
p. 102546

Abstract

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Glioblastoma is the most common malignant primary brain tumor with poor prognosis and low long-term survival rate. Here we described the production of an iPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 24-year-old female gliocytoma patient. The PBMCs were reprogrammed by the non-integrating Sendai Virus with human OKSM (OCT3/4, SOX2, KLF4, and c-MYC) transcription factors. The generated iPSCs were positive for pluripotent stem cell markers demonstrated by immunocytochemistry. Besides, they displayed a normal karyotype and had the potential to differentiate spontaneously three germ layers in vitro. Our model might be useful in studying the pathogenesis of gliocytoma patients.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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