Lipase-Assisted Synthesis of Alkyl Stearates: Optimization by Taguchi Design of Experiments and Application as Defoamers
Enoch Olvera-Ureña,
Jorge Lopez-Tellez,
M. Monserrat Vizueto,
J. Guadalupe Hidalgo-Ledezma,
Baltazar Martinez-Quiroz,
Jose A. Rodriguez
Affiliations
Enoch Olvera-Ureña
Area Academica de Quimica, Instituto de Ciencias Basicas e Ingenieria, Universidad Autonoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma 42184, Hidalgo, Mexico
Jorge Lopez-Tellez
Area Academica de Quimica, Instituto de Ciencias Basicas e Ingenieria, Universidad Autonoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma 42184, Hidalgo, Mexico
M. Monserrat Vizueto
Soluciones Quimicas Globales, S. de R.L.M.I., Rio Industrial 210, 47, Mineral de la Reforma 42186, Hidalgo, Mexico
J. Guadalupe Hidalgo-Ledezma
Soluciones Quimicas Globales, S. de R.L.M.I., Rio Industrial 210, 47, Mineral de la Reforma 42186, Hidalgo, Mexico
Baltazar Martinez-Quiroz
Soluciones Quimicas Globales, S. de R.L.M.I., Rio Industrial 210, 47, Mineral de la Reforma 42186, Hidalgo, Mexico
Jose A. Rodriguez
Area Academica de Quimica, Instituto de Ciencias Basicas e Ingenieria, Universidad Autonoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma 42184, Hidalgo, Mexico
The present work proposes the optimization of enzymatic synthesis of alkyl stearates using stearic acid, alkyl alcohols (C1-OH, C2-OH, C4-OH, C8-OH and C16-OH) and Candida rugosa lipase by a L9 (34) Taguchi-type design of experiments. Four variables were evaluated (reaction time, temperature, kU of lipase and alcohol:stearic acid molar ratio), ensuring that all variables were critical. In optimal conditions, five stearates were obtained with conversions > 90%. The obtained products were characterized by nuclear magnetic resonance (NMR). Additionally, the defoaming capacity of the five stearates was evaluated, obtaining better performance for the compound synthesized from C8-OH alcohol.
