Effect of Thawing Procedure and Thermo-Resistance Test on Sperm Motility and Kinematics Patterns in Two Bovine Breeds
Juan M. Solís,
Francisco Sevilla,
Miguel A. Silvestre,
Ignacio Araya-Zúñiga,
Eduardo R. S. Roldan,
Alejandro Saborío-Montero,
Anthony Valverde
Affiliations
Juan M. Solís
Animal Reproduction Laboratory, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica
Francisco Sevilla
Animal Reproduction Laboratory, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica
Miguel A. Silvestre
Department of Cellular Biology, Functional Biology and Physical Anthropology, University of Valencia, Campus Burjassot, C/Dr Moliner, 50, 46100 Valencia, Spain
Ignacio Araya-Zúñiga
Animal Reproduction Laboratory, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica
Eduardo R. S. Roldan
Department of Biodiversity and Evolutionary Biology, National Museum of Natural Sciences, Spanish National Research Council (CSIC), 28006 Madrid, Spain
Alejandro Saborío-Montero
Alfredo Volio Mata Experimental Station, Faculty of Agri-Food Sciences, University of Costa Rica, Cartago 30304, Costa Rica
Anthony Valverde
Animal Reproduction Laboratory, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica
This investigation aimed to analyze the effect that thawing time and temperature in combination with a termo-resistance test had on straws from dairy bulls used for artificial insemination (AI) on semen motility and kinematic variables measured with CASA systems. Eight animals of Holstein and Jersey breeds were used, and nine frozen-thawed semen doses per animal were analyzed for each breed. Three temperatures (35, 37, and 40 °C) and three thawing times (35, 40, and 45 s) were evaluated using a factorial design. Motility and kinematic patterns were analyzed using CASA-mot (Computer-Assisted Semen Analysis of motility) technology at different post-thawing times (0.5, 1, and 2 h). Sperm motility in Jersey bulls was higher (p p p p p < 0.05) as the post-thaw period was prolonged. The study showed differences in sperm quality between Holstein and Jersey breeds, influenced by cryopreservation, thawing, and post-thawing incubation. Thawing at 37 °C for 30 s was considered optimal in relation to sperm motility. In addition, a decrease in sperm quality was observed as post-thawing time increased.
