Nature Communications (Jan 2021)
The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus
- Mohammad M. Karimi,
- Ya Guo,
- Xiaokai Cui,
- Husayn A. Pallikonda,
- Veronika Horková,
- Yi-Fang Wang,
- Sara Ruiz Gil,
- Gustavo Rodriguez-Esteban,
- Irene Robles-Rebollo,
- Ludovica Bruno,
- Radina Georgieva,
- Bhavik Patel,
- James Elliott,
- Marian H. Dore,
- Danielle Dauphars,
- Michael S. Krangel,
- Boris Lenhard,
- Holger Heyn,
- Amanda G. Fisher,
- Ondřej Štěpánek,
- Matthias Merkenschlager
Affiliations
- Mohammad M. Karimi
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Ya Guo
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Xiaokai Cui
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Husayn A. Pallikonda
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Veronika Horková
- Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences
- Yi-Fang Wang
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Sara Ruiz Gil
- CNAG-CRG, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)
- Gustavo Rodriguez-Esteban
- CNAG-CRG, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)
- Irene Robles-Rebollo
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Ludovica Bruno
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Radina Georgieva
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Bhavik Patel
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- James Elliott
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Marian H. Dore
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Danielle Dauphars
- Department of Immunology, Duke University Medical Center
- Michael S. Krangel
- Department of Immunology, Duke University Medical Center
- Boris Lenhard
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Holger Heyn
- CNAG-CRG, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)
- Amanda G. Fisher
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- Ondřej Štěpánek
- Laboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of Sciences
- Matthias Merkenschlager
- MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
- DOI
- https://doi.org/10.1038/s41467-020-20306-w
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 14
Abstract
Developing T cells commit to either CD4/helper or CD8/cytotoxic lineage in the thymus, but how CD4 and CD8 coreceptors and TCR signaling dictate this selection process is still unclear. Here the authors use single cell RNA sequencing of mouse thymocytes to show that, in selection intermediates, TCR signaling strength informs coreceptor expression timing.
