Interaction of serum lipoproteins with the hydroperoxide of methyl linoleate*

Abstract

The interaction of serum lipoproteins with the hydroperoxide of methyl linoleate was studied by means of analytical ultracentrifugal or paper electrophoretic analysis. The results indicated that lipohydroperoxide seemed to have a marked effect on the stability of low density or β-lipoproteins. The hydroperoxide of methyl linoleate extensively denatured the isolated low density lipoproteins. An initial or primary association of the hydroperoxide of methyl linoleate with low density lipoproteins was noted prior to its denaturation. Furthermore, the results indicated that the hydroperoxide of methyl linoleate selectively or preferentially denatured β-lipoproteins and not other serum lipoproteins or protein constituents. In vivo studies indicated that the hydroperoxide of methyl linoleate inhibited lipid absorption. Although some lipohydroperoxide was absorbed from the intestinal tract when diluted with methyl linoleate, it is not known whether an exogeneous source of lipohydroperoxide can contribute to the in vivo denaturation or degradation of β-lipoproteins.