Co-administration of Hibiscus sabdariffa linn and Daflon-500® mitigates weight loss, hyperglycemia, hematological and oxidative changes in male rats with type-2 diabetes

Abstract

Background: Diabetes mellitus is a complex metabolic disorder associated with persistent hyperglycemia and metabolic imbalance. Flavonoid compounds possess notable qualities in mitigating diabetes and its complications. Aim: Hibiscus sabdariffa linn and daflon-500® were assessed for their effects on body weight, blood glucose/indices, and oxidative changes in type-2 diabetes rats over 28 days. Methods: 35 male rats were randomly divided into seven groups (n = 5). Diabetes mellitus was induced in groups 2–7 via a single intraperitoneal injection of streptozotocin (STZ; 55mg/kg). Group 1(DW) received distilled water (2mL/kg) only. Group 2 (STZ; untreated), group 3 {MET; metformin (Glucophage)}, group 4 {HS; Hibiscus sabdariffa (400mg/kg)}, group 5 {DAF; daflon-500® (1000mg/kg)}, group 6 {DAF+HS; daflon-500® (1000mg/kg) and Hibiscus sabdariffa (400mg/kg)}, group 7 {DAF+MET+HS; daflon-500® (1000mg/kg), metformin (Glucophage), and Hibiscus sabdariffa (400mg/kg)}. Body weight and blood glucose were assessed fortnightly. Blood and hepatic/pancreatic tissues were collected for hematological and antioxidant testing. Results: Groups 2–7 revealed a significant (P < 0.05) decrease in body weight on day 14 compared to the increased growth rate observed on day 28 (Group 7).Blood glucose levels significantly increased on days 1 and 14 in groups 2–7 but declined on day 28 in groups 4–7. Significantly higher packed cell volume, hemoglobin levels, red blood cell counts, lymphocytes, and mean corpuscular hemoglobin were observed in groups 5–7. Platelet counts were higher in Groups 2 and 5. Untreated group 2 exhibited considerably reduced catalase activity, glutathione, and malondialdehyde concentrations compared to the treated groups. Conclusion: Hibiscus sabdariffa linn and Daflon-500® expressed substantial hypoglycemic, antioxidant, and hemato-protective effects.

Keywords

• Diabetes mellitus
• Body weight
• Blood glucose
• Oxidative stress
• Daflon-500®
• Hibiscus sabdariffa