Optimization of the CYP inhibition assay using LC-MS/MS

Muhammad Asyraf Abduraman; Nor Hidayah Mustafa; Nik Soriani Yaacob; Azimah Amanah; Mei Lan Tan

MethodsX (Jan 2022)

Optimization of the CYP inhibition assay using LC-MS/MS

Muhammad Asyraf Abduraman,
Nor Hidayah Mustafa,
Nik Soriani Yaacob,
Azimah Amanah,
Mei Lan Tan

Affiliations

Muhammad Asyraf Abduraman: School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia
Nor Hidayah Mustafa: Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia
Nik Soriani Yaacob: School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia
Azimah Amanah: Malaysian Institute of Pharmaceuticals & Nutraceuticals (IPharm), National Institutes of Biotechnology Malaysia (NIBM), Pulau Pinang 11700, Malaysia
Mei Lan Tan: School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia; Corresponding author.

Journal volume & issue: Vol. 9
p. 101827

Abstract

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The data presented in this article are related to the research article entitled “Cytochrome P450 inhibition activities of non-standardized botanical products” [1], in which the possible CYP inhibitory properties of botanical products were investigated. This article describes the optimization and bioanalytical method validation of the CYP (Cytochrome P450 inhibition assay) inhibition assays, namely, phenacetin O-deethylase assay, testosterone 6β-hydroxylase assay, felodipine dehydrogenase assay and midazolam 1’-hydroxylase assay using LC-MS/MS.

Cytochrome P450 inhibition assay

Published in MethodsX

ISSN: 2215-0161 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science
Website: http://www.journals.elsevier.com/methodsx/

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